MAP70 proteins
In a recent study, we could show that MICROTUBE ASSOCIATED PROTEIN 70-5 (MAP70-5) contributes to spatial accommodating responses in the endodermis during lateral root formation in Arabidopsis (Stöckle et al., 2022, PMID: 35138892).
We are now characterization also the other 4 members of the MAP70 family of Arabidopsis and MAP70 proteins for other species.
MAP70-5 is required for spatial accommodation responses in the endodermis. (A) lateral root density in the LRDZ of WT compared to map70-5-c1 and map70-5-c2. For each line, Wilcoxon rank sum test with continuity correction was performed to compare LR densities. (B) Staging of LRs reveals an accumulation of stage I LRP in the map70-5-c1 and map70-5-c2 compared to WT roots. Pearson’s chi-square test with Yates’ continuity correction was used to assess whether LRP distribution is independentin the map70-5 mutants. (C and D) Single confocal planes showing LRP morphology in WT and map70-5-c1 through stages I to IV visualized by the plasma membrane marker UBQ10pro::EYFP:NPSN12. Contours of endodermal cells overlying the LRP are highlighted in green (C) under control conditions and pink (D) when PHS1ΔP was induced. (E) Maximum projection of confocal z-stacks showing CMTs on the inner and outer side of WT and map70-5-c1 endodermal cells. (F) CMT orientation (0° to 90°, respec-tive to the long axis of the cell). (G) CMT isotropy on the inner and outer side (a.u., 0 = no order and 1 = order). Scale bars, 10 µm. Comparison between samples was per-formed using two-way ANOVA and post hoc multiple comparisons with Tukey’s test. Samples with identical letters do not significantly differ (a = 0.05). Figure from Stöckle et al., 2022, PMID: 35138892.