BÙI Xuân Thái
ORCID: 0009-0004-4172-5785
Research description:
Auxin-regulated intercellular communication during lateral root formation
My project aims at better understanding of spatial accommodation responses and cell communication via the phytohormone auxin during lateral root formation in Arabidopsis thaliana. I characterized the auxin responses in a spatial accommodation mutant using auxin treatment assay combined with microscopy. Aside from that, a gene expression atlas specific to the endodermis and the pericycle in that mutant and the wild type generated using TRAP-seq method was analyzed to determine the candidate regulators of endodermal spatial accommodating responses, which would be functionally confirmed using a combination of molecular genetics and live cell imaging. In parallel, another cell type-specific transcriptomic dataset was created utilizing a synthetic convex auxin and its complementarily recognized engineered auxin receptor (cvxIAA – ccvTIR1 system) to further dissect the contribution of each cell layer into spatial accommodation. The results of this project will not only broaden the existing knowledge on plant spatial accommodation and lateral root development, but could also be potentially useful for future breeding programs for new crop varieties to efficiently mitigate climate change impacts.
Auxin treatments deform morphology of lateral root primordia in CASP1pro::shy2-2. 5-day old seedlings of Col-0 and CASP1pro::shy2-2 were treated with IAA, NAA and 2,4-D at 1 μM for up to 48 hours. Roman numerals, dashed lines and arrowheads indicate LRP stages and positions in Col-0 (cyan) and CASP1pro::shy2-2 (yellow). Asterisks indicate uncontrolled cell division. Scale bar = 20 μm. (unpublished)
Short CV:
| 2020 – now | Doctoral Assistant LBMC lab, University of Neuchâtel, Switzerland Supervisor: Dr. Joop VERMEER |
| 2019, Mar – Aug | Intern, “Cell biology of potassium transport systems in rice” TICER team, IPSiM Joint Research Unit, Institut Agro Montpellier, France Supervisor: Dr. Doan-Trung LUU |
| 2018, Apr – Jul | Intern, “Role of jasmonate in root architecture regulation under stress in rice” CERES team, DIADE Joint Research Unit, IRD Montpellier, France Supervisor: Dr. Antony CHAMPION |
| 2017 – 2019 | MSc., Plant breeding (SEPMET) Institut Agro Montpellier, France |
| 2017, Apr – Aug | Intern, “Jasmonate response diversity to abiotic stress in rice” LMI-RICE2 International Joint Laboratory, Agricultural Genetics Institute, Vietnam Supervisor: Dr. Thi Mai Huong TO |
| 2016, Apr-Sep | Intern, “Nutrient limitation in relation to the growth and primary production of phytoplankton in Jeju coastal area” Korean Institute of Ocean Science and Technology, South Korea Supervisor: Dr. Sinjae YOO |
| 2013 – 2016 | BSc., Biotechnology (PMAB) University of Science and Technology of Hanoi, Vietnam |